The evolution of 3D-printing technology has transformed the landscape of regenerative medicine, particularly concerning the development of customizable bio-scaffolds for bone healing. This systematic review analyzes the progress, advantages, and clinical applications of 3D-printed bio-scaffolds within orthopedic fracture healing and orthodontic bone remodeling. Current evidence highlights their impactful role in achieving improved bone regeneration, infection control, and mechanical strength. Furthermore, the adaptability of scaffold architectures to complex anatomical sites offers new directions for highly personalized medicine. However, challenges regarding vascularization, scaffold degradation, and regulatory pathways remain present. The article concludes with insights for further research and translation into clinical practice.